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Stable isotope-labeling of DNA repair proteins, and their purification and characterization

โœ Scribed by Prasad T. Reddy; Pawel Jaruga; Bryant C. Nelson; Mark Lowenthal; Miral Dizdaroglu

Book ID
116829301
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
534 KB
Volume
78
Category
Article
ISSN
1046-5928

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For the application of multidimensional NMR spectroscopy to larger proteins, it would be useful to perform selective labeling of one of the 20 amino acids. For some amino acids, however, amino acid metabolism drastically reduces the efficiency and selectivity of labeling in in vivo expression system