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Cell-free production and stable-isotope labeling of milligram quantities of proteins

โœ Scribed by Takanori Kigawa; Takashi Yabuki; Yasuhiko Yoshida; Michio Tsutsui; Yutaka Ito; Takehiko Shibata; Shigeyuki Yokoyama

Book ID
117110224
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
364 KB
Volume
442
Category
Article
ISSN
0014-5793

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Cell-free synthesis and amino acid-selec
Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis
โœ Takanori Kigawa; Yutaka Muto; Shigeyuki Yokoyama ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 536 KB

For the application of multidimensional NMR spectroscopy to larger proteins, it would be useful to perform selective labeling of one of the 20 amino acids. For some amino acids, however, amino acid metabolism drastically reduces the efficiency and selectivity of labeling in in vivo expression system