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Stable, High-Level Expression of a Type I Antifreeze Protein inEscherichia coli

โœ Scribed by Robert G. Solomon; Rudi Appels


Book ID
115645461
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
741 KB
Volume
16
Category
Article
ISSN
1046-5928

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## A thermophilic Bacillus sp. producing xylose (glucose) isomerase has been isolated. Its xy/A gene when cloned in Escherichia co/i and expressed gave 37.5 and 12.8 units/ rng, protein respectively for xylose and glucose isomerase activities at 85 C. A single heat treatment of the crude extract p