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High-level expression of alacZgene from a bacterial artificial chromosome inEscherichia coli

โœ Scribed by T.-S. Chang; W.-J. Wu; H.-M. Wan; T.-R. Shiu; W.-T. Wu


Publisher
Springer
Year
2003
Tongue
English
Weight
242 KB
Volume
61
Category
Article
ISSN
1432-0614

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High level expression of a thermostableB
โœ Liao Wuxiang; Kandiah Jeyaseelan ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 348 KB

## A thermophilic Bacillus sp. producing xylose (glucose) isomerase has been isolated. Its xy/A gene when cloned in Escherichia co/i and expressed gave 37.5 and 12.8 units/ rng, protein respectively for xylose and glucose isomerase activities at 85 C. A single heat treatment of the crude extract p