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Stabilization of Bacillus stearothermophilus neutral protease by introduction of prolines

✍ Scribed by F. Hardy; G. Vriend; O.R. Veltman; B. van der Vinne; G. Venema; V.G.H. Eijsink


Book ID
115928536
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
525 KB
Volume
317
Category
Article
ISSN
0014-5793

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## Abstract Engineered extremely thermostable variants of the thermolysin‐like protease from __Bacillus stearothermophilus__ possessing an introduced disulfide bond G8C/N60C (double mutant, DM) and six additional amino acid substitutions in the exposed loop region 56–69 (Boilysin, BLN) have been pr