Addition of a methyl group changes both the catalytic velocity and thermostability of the neutral protease from Bacillus stearothermophilus

The stability of engineered thermostable

The stability of engineered thermostable neutral proteases from Bacillus stearothermophilus in organic solvents and detergents

✍ Johanna Mansfeld; Renate Ulbrich-Hofmann 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 159 KB 👁 1 views

## Abstract Engineered extremely thermostable variants of the thermolysin‐like protease from __Bacillus stearothermophilus__ possessing an introduced disulfide bond G8C/N60C (double mutant, DM) and six additional amino acid substitutions in the exposed loop region 56–69 (Boilysin, BLN) have been pr