The stability of engineered thermostable
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Johanna Mansfeld; Renate Ulbrich-Hofmann
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Article
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2006
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John Wiley and Sons
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English
β 159 KB
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## Abstract Engineered extremely thermostable variants of the thermolysinβlike protease from __Bacillus stearothermophilus__ possessing an introduced disulfide bond G8C/N60C (double mutant, DM) and six additional amino acid substitutions in the exposed loop region 56β69 (Boilysin, BLN) have been pr