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The effect of cavity-filling mutations on the thermostability of Bacillus stearothermophilus neutral protease

✍ Scribed by Eijsink, Vincent G.H.; Dijkstra, Bauke W.; Vriend, Gerrit; van der Zee, J.Rob; Vettman, Oene R.; van der Vinne, B.; van den Burg, B.; Kempe, S.; Venema, G.


Book ID
118054535
Publisher
Oxford University Press
Year
1992
Tongue
English
Weight
506 KB
Volume
5
Category
Article
ISSN
1741-0126

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## Abstract Engineered extremely thermostable variants of the thermolysin‐like protease from __Bacillus stearothermophilus__ possessing an introduced disulfide bond G8C/N60C (double mutant, DM) and six additional amino acid substitutions in the exposed loop region 56–69 (Boilysin, BLN) have been pr