Sperm antigen MSH27 participates in sperm-egg membrane fusion
β Scribed by Dazhong Zhuang; Xiangfen Song; Guojun Hu; Qingyuan Sun; Dayuan Chen
- Book ID
- 105728465
- Publisher
- Springer
- Year
- 1999
- Tongue
- English
- Weight
- 654 KB
- Volume
- 44
- Category
- Article
- ISSN
- 1001-6538
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In this study, we examined the potassium requirements for sperm-egg fusion in mouse. Zona-free mouse eggs prepared by the method described by Boldt and Wolf were inseminated with capacitated sperm in culture media containing 0-6 mM extracellular K+, and scored for penetration. Penetration of zona-fr
Effects of various sulfhydryl (SH)-depleting reagents on sperm-egg fusion were demonstrated. When sperm were treated with three plasma membrane-permeable SH-depleting reagents, N-ethyl-maleimide, sodium tetrathionate and 5,5Β΄-dithiobis (2-nitro-benzoic acid), the rates of cleavage of eggs were signi