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Spectrum of spontaneous mutations in liver tissue of lacI transgenic mice

✍ Scribed by Johan G. de Boer; Heather Erfle; David Walsh; James Holcroft; J. Scott Provost; Brenda Rogers; Kenneth R. Tindall; Barry W. Glickman

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 100 KB
Volume: 30
Category: Article
ISSN: 0893-6692
DOI: 10.1002/(sici)1098-2280(1997)30:3<273::aid-em5>3.0.co;2-m

No coin nor oath required. For personal study only.

✦ Synopsis

The advent of transgenic technology has greatly fa-deamination-directed mutation at methylated cytocilitated the study of mutation in animals in vivo. sine bases. The second most common class of muta-The Big Blue mouse system, transgenic for the lacI tions is G:C r T:A transversions. A significant clonal gene, permits not only the quantification of muta-expansion of mutants was found in several animals, tions in different tissues but also provides for the and this was used to make an approximate correcgeneration of in vivo-derived mutational spectra. tion of the mutant frequency such that the most con-This report details the sequence alterations of 348 servative estimate of mutation frequency is prespontaneous mutations recovered from the liver of sented. The establishment of this substantial data-6-8-week-old male Big Blue mice. The spectra re-base of spontaneous mutations in the liver of Big covered from two strains of mice, C57Bl/6 and Blue mice is intended to serve as a reference B6C3F1, were compared and found to be very simi-against which mutations recovered after treatment lar. The predominant mutations are G:C r A:T tran-can be compared.

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