Big Blue ® mice harbor a recoverable transgene in a lambda/LIZ shuttle vector. In the standard assay, in vivo mutations are measured in the bacterial lacI gene using a labor-intensive color plaque assay. Applying a simpler assay : Proc Natl Acad Sci USA 93:9073-9078], we measured mutations in the la
Genotoxicity of 3-methylcholanthrene in liver of transgenic Big Blue® mice
✍ Scribed by Bertrand Henri Rihn; Marie-Claire Bottin; Catherine Coulais; Raphaël Rouget; Nathalie Monhoven; Wlodzimierz Baranowski; Aléodjrodo Edorh; Gérard Keith
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 143 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0893-6692
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The advent of transgenic technology has greatly fa-deamination-directed mutation at methylated cytocilitated the study of mutation in animals in vivo. sine bases. The second most common class of muta-The Big Blue mouse system, transgenic for the lacI tions is G:C r T:A transversions. A significant c
We determined the spectrum of mutations in the lacI gene in the liver of Big Blueா transgenic mice after exposure to five daily doses of 2 mg/kg dimethylnitrosamine (DMN) at 3 and 6 weeks of age. This dose has been reported to increase the mutant frequency 9-fold when the animals are 3 weeks old. Th
Organ specificity in the lac/ mutant frequency (MF) induced by dimethylnitrosamine (DMN) was analyzed in lung, liver, kidney, bone marrow, urinary bladder, and testis of Big Blue@ mice. Cell proliferative activity was also analyzed in some of these tissues by immunohistochemical staining of prolifer
The antitumour effect of tea plant root extract (TRE) has been evaluated against a 3-methylcholanthrene (3-MC) induced solid tumour model in ICR mice. TRE inhibited the tumur weight and the tumours were found to be nonnecrotic. Superoxide dismutase (SOD), a free radical scavenger, in the sera of TRE
to the previously established Big Blue ® assay. Genomic DNA isolated from liver, spleen, and lung tissue of control or ethylnitrosourea (ENU)-treated Big Blue ® mice (100 mg/kg i.p., single dose) was packaged into phage (five animals, two packagings per DNA sample) which were simultaneously plated f