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Other transgenic mutation assays: Tissue specificity of spontaneous point mutations in λsupF transgenic mice

✍ Scribed by Eric G. Leach; Latha Narayanan; Pamela A. Havre; Edward J. Gunther; Toni M. Yeasky; Peter M. Glazer

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
498 KB
Volume
28
Category
Article
ISSN
0893-6692

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✦ Synopsis

Transgenic mice carrying multiple copies of a recoverable h phage shuttle vector carrying the supF mutation reporter gene (hsupF) were constructed for the purpose of studying mutagenesis in a whole animal. Spontaneous mutations in rescued supF target genes from mouse liver and skin were analyzed. The mutation frequency was similar in both tissues (in the range of 2 x 1 0-5), but the spectrum of point mutations was distinct, with transitions common in the skin and transversions more prominent in the liver (P = 0.01). These results may help to elucidate pathways of endogenous mutogenesis in vivo, and they illustrate potentially important tissuespecific differences in genetic instability. o 1996

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