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Spectrophotometric Determination of the Positional Specificity of Nonspecific and 1,3-Specific Lipases

✍ Scribed by Ricardo N. Farias; Mercé Torres; Ramon Canela

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
99 KB
Volume
252
Category
Article
ISSN
0003-2697

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✦ Synopsis

Using commercially available thiosubstrates, such as 2,3-dimercapto-1-propanol tributyrate, the regio-specificities of 1,3-specific and nonspecific lipases was confirmed. The spectrophotometric test is a simple, rapid, and convenient alternative method to those previously reported for the characterization of the positional specificities of new lipases.

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