Spectrophotometric determination of cysteine and cystine in urine
β Scribed by Chrastil, Joseph
- Book ID
- 127135812
- Publisher
- Royal Society of Chemistry
- Year
- 1990
- Tongue
- English
- Weight
- 267 KB
- Volume
- 115
- Category
- Article
- ISSN
- 0003-2654
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Biologically Important sulphydryl compounds, e g cysteme, were determined by treatmg the reductant tluol with copper(B)-neocuprom (NC) reagent m ammonium acetate-buffered medium, followed by measunng the absorbance of the copper(I)-Nc complex at 450 nm Cystme, d present m a rmxture Hnth cysteme, was
## Abstract A spectrophotometric method based on myeloperoxidase activity for the determination of leukocytes in urine is described. Red cells that may be found in urine samples were lysed by an ammonium chloride method. Leukocytes were then sedimented by centrifugation and lysed using Triton Xβ100