Spectrophotometric determination of cysteine and cystine in peptides and proteins
β Scribed by Chrastil, Joseph
- Book ID
- 117998542
- Publisher
- Royal Society of Chemistry
- Year
- 1989
- Tongue
- English
- Weight
- 563 KB
- Volume
- 114
- Category
- Article
- ISSN
- 0003-2654
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π SIMILAR VOLUMES
Cysteine and half-cystine in proteins and peptides can be determined by acid hydrolysis in the presence of dithiodiglycolic acid, derivatization with phenyl isothiocyanate, and HPLC separation of the phenylthiocarbamyl amino acids. The determination requires no additional sample handling, oxidation,
Biologically Important sulphydryl compounds, e g cysteme, were determined by treatmg the reductant tluol with copper(B)-neocuprom (NC) reagent m ammonium acetate-buffered medium, followed by measunng the absorbance of the copper(I)-Nc complex at 450 nm Cystme, d present m a rmxture Hnth cysteme, was