On the cysteine and cystine content of proteins
โ Scribed by Robert C. Fahey; John S. Hunt; Gayle C. Windham
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 330 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0022-2844
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๐ SIMILAR VOLUMES
Quantitative analysis for cysteinyl, cystinyl and tryptophyl residues, as well as those of the more stable amino acids, may be made on a single column of an amino acid analyzer after acid hydrolysis of either soluble or insoluble proteins. The procedure involves determination of cysteinyl residues a
So far, the cystine (disulfide) content of proteins has been routinely determined by indirect methods, i.e., cystines are first converted to more stable half-cystines prior to analysis. We present a study which demonstrates that the cystine content can be directly and accurately analyzed at low pico