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Single analysis for cysteine, cystine, and tryptophan in proteins

✍ Scribed by A.S. Inglis; D.T.W. McMahon; C.M. Roxburgh; H. Takayanagi

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 564 KB
Volume: 72
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90509-1

No coin nor oath required. For personal study only.

✦ Synopsis

Quantitative analysis for cysteinyl, cystinyl and tryptophyl residues, as well as those of the more stable amino acids, may be made on a single column of an amino acid analyzer after acid hydrolysis of either soluble or insoluble proteins. The procedure involves determination of cysteinyl residues as S-p-[4-pyridylethyl]cysteine, and cystinyl residues as S-sulfocysteine, after hydrolysis of the pyridylethyl-protein by 4 M methanesulfonic acid. The presence of tryptamine during hydrolysis was mandatory for quantitative tryptophan recovery and did not affect the subsequent determination of cystine as S-sulfocysteine.

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