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Spectrophotometric assay of clavulanic acid by reaction with imidazole

✍ Scribed by Bird, Albert E.; Bellis, Jeanne M.; Gasson, Brian C.


Book ID
119999388
Publisher
Royal Society of Chemistry
Year
1982
Tongue
English
Weight
489 KB
Volume
107
Category
Article
ISSN
0003-2654

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A spectrophotometric method for determination of bromopyruvate, based on the reaction with 2-nitro-S-thiobenzoic acid, is described. The reaction is complete in 30 min at room temperature in 0.1 M Tris-MES, 1 mM EDTA, pH 8.0. The method is sensitive to at least 1 x 10m5 M bromopyrnvate. Reagents are