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Spectrophotometric determination of bromopyruvate by reaction with 2-nitro-5-thiobenzoic acid

✍ Scribed by Shyun-long Yun; Clarence H. Suelter

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 276 KB
Volume: 85
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90240-3

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✦ Synopsis

A spectrophotometric method for determination of bromopyruvate, based on the reaction with 2-nitro-S-thiobenzoic acid, is described. The reaction is complete in 30 min at room temperature in 0.1 M Tris-MES, 1 mM EDTA, pH 8.0. The method is sensitive to at least 1 x 10m5 M bromopyrnvate. Reagents are stable, easy to prepare, and specific for P-halopyruvate. Bromopyruvate solutions must be prepared fresh daily. Solutions of bromopyruvate at pH 8.0 and 23°C have a half-life of 3 hr.

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