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Spectrophotometric determination of tryptophan by reaction with nitrous acid

✍ Scribed by Krishna K. Verma; Archana Jain; J. Gasparič

Book ID
118007779
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
431 KB
Volume
35
Category
Article
ISSN
0039-9140

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✍ Shyun-long Yun; Clarence H. Suelter 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 276 KB

A spectrophotometric method for determination of bromopyruvate, based on the reaction with 2-nitro-S-thiobenzoic acid, is described. The reaction is complete in 30 min at room temperature in 0.1 M Tris-MES, 1 mM EDTA, pH 8.0. The method is sensitive to at least 1 x 10m5 M bromopyrnvate. Reagents are