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Spectrofluorimetric determination of malic acid with β-naphthol

✍ Scribed by Gary D. Christian; John R. Moody

Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
500 KB
Volume
41
Category
Article
ISSN
0003-2670

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✦ Synopsis

In 1932, EacnrwE 1 developed a fluorirnctric qualitative test for malic acid based upon its reaction with P-naphthol in concentrated sulfuric acid. LErNIscEn AND KATZ2 later reported a quantitative method for rnalic acid based upon this fluorescence which eliminated the interference of citric acid found when resorcinol is used as the reagenta. They were able to determine r-30 ~6 of malic acid in apple juice. Citric and succinic acids did not interfere and tart&c acid showed a small interference when present in amounts equal to the malic acid present.

The present paper reports a dctailcd investigation of the optimum conditions for this reaction and for measuring the fluorescence intensity employing a spectrophotofluorimetcr.

As little as 0.1~6 or 0.002 p.p.m. of malic acid can be determined.

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