Spectrofluorimetric determination of bile acid using a europium-doxycycline probe

✍ Scribed by Ting Wang; Xiaolei Wang; Chongqiu Jiang

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 139 KB
• Volume: 21
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.20137

✦ Synopsis

Abstract

A new spectrofluorimetric method was developed for the determination of trace amounts of bile acid (BA). Using europium ion (Eu^3+^)‐doxycycline (DC) as a fluorescent probe, in a buffer solution of pH=7.0, BA can remarkably reduce the fluorescence intensity of the DC‐Eu^3+^ complex at λ=612 nm; the reduced fluorescence intensity of the Eu^3+^ is proportional to the concentration of BA. Optimum conditions for the determination of BA were also investigated. The linear range and detection limit for the determination of BA were 5.0 × 10^−8^ mol/L to 5.5 × 10^−7^ mol/L and 1.1 × 10^−8^ mol/L, respectively. This method is practical and relatively free of interference from coexisting substances, and can be successfully applied to assess BA in serum samples. J. Clin. Lab. Anal. 21:207–212, 2007. © 2007 Wiley‐Liss, Inc.