Spectral phase interference: A tool for the study of adsorption processes on solid surfaces

✍ Scribed by Kusterer, Jan ;Bakowsky, Udo ;Keusgen, Michael

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 419 KB
• Volume: 208
• Category: Article
• ISSN: 0031-8965
• DOI: 10.1002/pssa.201001223

✦ Synopsis

Abstract

Spectral phase interference (SPI) has attracted considerable attention during last years as method for biomolecular interaction analysis (BIA). The advantages of this method are real time measurements independently from the refraction index of the investigated solution. Therefore, this method was applied for evaluating the influence of mouth rinsing solutions like chlorhexidine digluconate (CHX)‐solutions on the pellicle formation upon a tooth model. The tooth model was a based glass chip, type ‘K0’. The surface of this activated glass chip is comparable to the surface of the enamel of a tooth. After surface activation, saliva was applied in order to deposit a pellicle layer. The pellicle is the primary protein layer formed on the surface of a tooth. This layer has the function to prevent a direct contact of bacteria with the tooth surface. As a disadvantage, this layer is the docking point and primary nutrition reservoir for bacteria forming the plaque. Analysing the interaction of this bio layer with mouth rinsing solutions is essential to evaluate effects on the formation of plaque. In this investigation, evidences for positive effects of CHX‐solutions and amino fluorides on the formation of the pellicle have been found. Treatment with these solutions resulted in a dose dependent enhanced thickness of the pellicle. It can be assumed, that these modified pellicle layers are more resistant against bacteria attack.