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Specific removal of anti-red cell blood group activity from anti-HLA lymphocytotoxic sera and purification of anti-A and anti-B antibodies with lymphocytotoxic activity

✍ Scribed by Marietta Bernoco; Domenico Bernoco; John Danilovs; Paul I. Terasaki; Rosella Mollicone; Rafael Oriol


Book ID
115093714
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
325 KB
Volume
26
Category
Article
ISSN
0001-2815

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A simple method for purification of anti
✍ Katsumata, Y. ;Sato, M. πŸ“‚ Article πŸ“… 1985 πŸ› Springer-Verlag 🌐 English βš– 209 KB

A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human erythrocytes is described. Specific antibodies were first absorbed with the corresponding cells, then eluted by heating at 53Β°-55Β°C for 15 min. The method is simple and highly efficient with a fair reco