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A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human red blood cells

✍ Scribed by Katsumata, Y. ;Sato, M.

Publisher: Springer-Verlag
Year: 1985
Tongue: English
Weight: 209 KB
Volume: 95
Category: Article
ISSN: 1437-1596
DOI: 10.1007/bf00201074

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✦ Synopsis

A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human erythrocytes is described. Specific antibodies were first absorbed with the corresponding cells, then eluted by heating at 53°-55°C for 15 min. The method is simple and highly efficient with a fair recovery of 15.6%-34.4%.

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