Specific binding of radioiodinated human GM-CSF to the blast cells of acute myeloblastic leukemia
β Scribed by Ikuo Murohashi; Shuji Tohda; Toshiya Suzuki; Kaoru Nagata; Yoko Yamashita; Nobuo Nara
- Book ID
- 113239120
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 524 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0145-2126
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Recombinant hemopoietic colony-stimulating factors (CSFs), including GM-CSF, G-CSF and IL-3, have been shown to be effective stimulators of both self-renewal and terminal differentiation of blast stem cells in acute myeloblastic leukemia (AML). We have examined the activity of a fourth growth factor
Myelopoietic growth factors are known to influence the growth in culture of malignant blast cells from human Acute Myeloblastic Leukemia (AML). We have used cDNA clones for the factor CSF-1 and its receptor fms to study DNA and RNA from the blasts of 25 AML patients. The CSF-1 gene was always in the
## Abstract Blast cells from patients with Acute Myeloblastic Leukemia (AML) were separated according to cell size using velocity sedimentation under unit gravity. Fractions obtained in this way were plated in methyl cellulose with a growth stimulator present in media conditioned by leukocytes in t