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SOn-column Protein Refolding for Crystallization

✍ Scribed by Natalia Oganesyan; Sung-Hou Kim; Rosalind Kim


Book ID
106442363
Publisher
Springer Netherlands
Year
2005
Tongue
English
Weight
228 KB
Volume
6
Category
Article
ISSN
1345-711X

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✍ Eliana De Bernardez Clark πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 64 KB

Inclusion body refolding processes are poised to play a major role in the production of recombinant proteins. Improving renaturation yields by minimizing aggregation and reducing chemical costs are key to the industrial implementation of these processes. Recent developments include solubilization me

Design analysis for refolding monomeric
✍ Nicholas Kotlarski; Brian K. O'Neill; Geoffrey L. Francis; Anton P. J. Middelber πŸ“‚ Article πŸ“… 1997 πŸ› American Institute of Chemical Engineers 🌐 English βš– 894 KB

Renaturation of protein expressed as inclusion bodies within Escherichia coli is a key step in many bioprocesses. Operating conditions for the refolding step dramatically affect the amount of protein product recovered, and hence profoundly influence the process economics. The first systematic compar