SOn-column Protein Refolding for Crystallization
β Scribed by Natalia Oganesyan; Sung-Hou Kim; Rosalind Kim
- Book ID
- 106442363
- Publisher
- Springer Netherlands
- Year
- 2005
- Tongue
- English
- Weight
- 228 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1345-711X
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π SIMILAR VOLUMES
Inclusion body refolding processes are poised to play a major role in the production of recombinant proteins. Improving renaturation yields by minimizing aggregation and reducing chemical costs are key to the industrial implementation of these processes. Recent developments include solubilization me
Renaturation of protein expressed as inclusion bodies within Escherichia coli is a key step in many bioprocesses. Operating conditions for the refolding step dramatically affect the amount of protein product recovered, and hence profoundly influence the process economics. The first systematic compar