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Human prion proteins expressed in Escherichia coli and purified by high-affinity column refolding

✍ Scribed by Ralph Zahn; Christine von Schroetter; Kurt Wüthrich


Book ID
117108413
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
571 KB
Volume
417
Category
Article
ISSN
0014-5793

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Recombinant human interleukin-6 (hIL-6), a pleiotropic cytokine containing two intramolecular disulfide bonds, was expressed in Escherichia coli as an insoluble inclusion body, before being refolded and purified in high yield providing sufficient qualities for clinical use. Quantitative reconstituti