𝔖 Bobbio Scriptorium
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Protein refolding for industrial processes

✍ Scribed by Eliana De Bernardez Clark

Book ID
104361562
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
64 KB
Volume
12
Category
Article
ISSN
0958-1669

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✦ Synopsis

Inclusion body refolding processes are poised to play a major role in the production of recombinant proteins. Improving renaturation yields by minimizing aggregation and reducing chemical costs are key to the industrial implementation of these processes. Recent developments include solubilization methods that do not rely on high denaturant concentrations and the use of high hydrostatic pressure for simultaneous solubilization and renaturation.

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Renaturation of protein expressed as inclusion bodies within Escherichia coli is a key step in many bioprocesses. Operating conditions for the refolding step dramatically affect the amount of protein product recovered, and hence profoundly influence the process economics. The first systematic compar