Thin-layer chromatography of keto acid 2,4-dinitrophenylhydrazones was carried out on plate coated with silica gel and sodium bicarbonate. A clear separation was obtained between most of the various keto acid hydrazones. The hydrazone was eluted from the plate with methanol containing 1% concentrate
Some technical improvements in the paper chromatography of keto acid 2,4-dinitrophenylhydrazones
β Scribed by A.S. Saini
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 292 KB
- Volume
- 26
- Category
- Article
- ISSN
- 1873-3778
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## After conversion to 2,4-dinitrophenylhydrazones, keto acids in biological fluids were extracted into isopropyl ether followed by NaHCO, and finally into ethyl acetate.
For characterizing keto acids, paper chromatography of their DNPHa is most widely used irrespective of the disadvantages (1)) some of which are as follows: According to the methods reported before 1962, the DNPH formed by the usual method were extracted from water solution with an organic solvent su
Using a uniselector or multi-way switch and electric clock, with electrodes placed at measured intervals down the paper, solvent run times may be recorded. This has been of considerable use when working at o".