The detection of keto acids in biological fluids by two dimensional paper chromatography of their 2,4-dinitrophenylhydrazones
β Scribed by R.F. Coward; P. Smith
- Publisher
- Elsevier Science
- Year
- 1968
- Tongue
- English
- Weight
- 578 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
β¦ Synopsis
After conversion
to 2,4-dinitrophenylhydrazones, keto acids in biological fluids were extracted into isopropyl ether followed by NaHCO, and finally into ethyl acetate.
π SIMILAR VOLUMES
A column ion-exchange chromatographic method for the determination of 2-carboxy thiazolidine-4-carboxylic acid (TDCA) in blood and urine is described. After elimination of endogenous thiols, alkaline hydrolysis of the compound yields cysteine which is determined spectrophotometrically. The described
The use of high-performance liquid chromatography in the reversed-phase mode coupled to a spectrophotofluorimetric detector allowed the selective detection of acidic indoles possessing a C-3 methylene side chain, following the reaction of a purified plant extract with acetic anhydride. Of these acid