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Some recent studies on the composition and structure of nucleic acids

โœ Scribed by Chargaff, Erwin


Publisher
Wiley (John Wiley & Sons)
Year
1951
Tongue
English
Weight
712 KB
Volume
38
Category
Article
ISSN
0095-9898

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โœฆ Synopsis


The first question that must be answered before the determination of the structure of nucleic acids can be carried out in earnest is: how much of what do they contain? It seemed to us that procedures suitable for this purpose had been lacking in the past and that no real progress was possible, unless the necessary analytical groundwork was laid. Following the demonstration that the excellent method of chromatography on filter paper (Consden, Gordon and Martin, '44) could be applied t o the separation of purines (Vischer and Chargaff, '47), procedures for the separation, in minute amounts, of the nitrogenous nucleic acid constituents and for their identification and quantitative estimation by spectrophotometry were elaborated (purines and pyrimidines :


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