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Some modifications in the procedure of direct sequencing of PCR amplified 16S rDNA

✍ Scribed by M Dorsch; E Stackebrandt

Book ID
119126211
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
770 KB
Volume
16
Category
Article
ISSN
0167-7012

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A number of different procedures have been developed for direct sequence analysis of PCR products. These methods rely on the cumbersome isolation of specific PCR products from agarose gels or the production of single-stranded template DNAs. In the approach presented here, we describe primers for the