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Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification

✍ Scribed by A. Hiraishi


Book ID
115271945
Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
656 KB
Volume
15
Category
Article
ISSN
0266-8254

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A number of different procedures have been developed for direct sequence analysis of PCR products. These methods rely on the cumbersome isolation of specific PCR products from agarose gels or the production of single-stranded template DNAs. In the approach presented here, we describe primers for the