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Direct Sequencing of DNA Fragments Amplified with Single Primers by Polymerase Chain Reaction without Cloning

โœ Scribed by Masayoshi Iizuka; Yuki Sugiyama; Shigeru Iida; Takao Sekiya


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
177 KB
Volume
241
Category
Article
ISSN
0003-2697

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Amplification of human genomic DNA seque
โœ Liuying Luo; Eleftherios P. Diamandis ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 157 KB ๐Ÿ‘ 1 views

We present two examples of exponential nucleic acid amplification with the polymerase chain reaction (PCR) in the presence of only one amplification primer. Cloning and sequencing of the PCR products generated by amplification of human genomic DNA revealed that the amplified sequence contained only