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Direct sequencing of double-stranded polymerase chain reaction-amplified 16S rDNA

✍ Scribed by Beatrix Both; Guido Krupp; Erko Stackebrandt

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
999 KB
Volume
199
Category
Article
ISSN
0003-2697

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✦ Synopsis

A number of different procedures have been developed for direct sequence analysis of PCR products. These methods rely on the cumbersome isolation of specific PCR products from agarose gels or the production of single-stranded template DNAs. In the approach presented here, we describe primers for the amplification of 16-S rDNA and a simple preparation of PCR product for sequencing.

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