Some characteristics of Ca2+uptake by yeast cells
✍ Scribed by Miguel Borbolla; Antonio Peña
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 633 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0022-2631
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The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor exogenous DNA upon Ca2+ treatment. At saturating DNA concentrations the cells were capable to take up irreversibly about 6-10(8) dalton of donor DNA per cell (if to
## Abstract Intracellular Ca^2+^ sequestration was studied by determining ^45^Ca^2+^ uptake by saponin‐permeabilized Swiss 3T3 mouse fibroblasts. Uptake was enhanced by addition of ATP and was dependent on the concentrations of ATP and saponin. ADP and UTP also enhanced uptake, but less than did AT