Ethidium bromide blocks ascus formation in the yeast Saccharomyces cerevisiae. This may mean that the presence of the mitochondrial genome is required for sporulation in this organism.
Effect of ethidium bromide on Ca2+uptake by yeast
✍ Scribed by Antonio Peña
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 692 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0022-2631
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An intermediate in the ethidium bromide (EB) induced petite mutation pathway may be destabilized by daylight light to cause a reversion to the normal grande phenotype. Starved cells preincubated in the dark for up to 6 h with 100 microgram/ml EB could be reverted to grandes after one hour of light e
## Abstract A self‐consistent thermodynamic characterization of the binding of ethidium to yeast phenylalanine‐specific tRNA at 25°C, pH 7.0, in 11 n__M__ MgCl~2~, 375 n__M__ NaCl, and 25 m__M__ sodium phosphate has been obtained. Two ethidium molecules bind per tRNA under these conditions. The str