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Enhanced uptake of donor DNA by Ca2+ treated Escherichia coli cells

✍ Scribed by Sabelnikov, A.G. ;Avdeeva, A.V. ;Ilyashenko, B.N.

Publisher
Springer
Year
1975
Tongue
English
Weight
444 KB
Volume
138
Category
Article
ISSN
0026-8925

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✦ Synopsis

The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor exogenous DNA upon Ca2+ treatment. At saturating DNA concentrations the cells were capable to take up irreversibly about 6-10(8) dalton of donor DNA per cell (if to assume that all the cells are competent). About 75% of this DNA was found to be attached to the cytoplasmic membrane. Employing Poisson approximation for distribution of the donor-marker DNA molecules on recipient cells it was found that the efficiency of a marker recombination and expression is about 2-10(-5). The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor 0exogenous

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