Some aspects of hybridoma cell cultivation

✍ Scribed by H. Graf; K. Schügerl

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 733 KB
• Volume: 35
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00184681

✦ Synopsis

Two h y b r i d o m a cell lines were cultivated in an indirectly aerated 10-1 reactor in batch, fed-batch and continuous (perfusion) operations and in spinner flasks. The m e d i u m in the reactor was sampled either by an aseptic cross-flow filtration module integrated into a loop or by an in-situ tubular filter. The glucose concentration was monitored by an on-line flow injection analyser and the a m m o n i a concentration by an ion-selective electrode. Since the m e m b r a n e transmission o f the high-molecular c o m p o n e n t s decreased during cultivation, the product, a m o n o c l o n a l antibody, was enriched in the reactor. During cultivation, the concentrations o f cells, viable cells, glucose, lactase, acetate, citrate, ammonia, urea, amino acids, proteins, and m o n o c l o n a l antibodies were determined off-line. The specific growth rate, specific production, and consumption rates o f the m e d i u m components were influenced considerably by the m e d i u m composition, especially by the type and a m o u n t o f serum used.