Poly(ethylenimine)-reinforced liquid-core capsules for the cultivation of hybridoma cells
✍ Scribed by Yuan-Lung Hsu; I-Ming Chu
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 851 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
The mechanical stability of liquid-core alginate-poly-L-lysine (PLL) capsules used for encapsulation of hybridoma cells can be greatly enhanced by the inclusion of poly(ethy1enimine) (PEI) in the hardening solution containing calcium chloride. The PEI can also reinforce the PLL-coated carboxymethylcelluose liquid-core capsules. The cultivation of murein hybridoma CT04 in these two capsules was carried out. Cell concentrations higher than lo* cells/mL per capsule were obtained with ca. 80% of the specific antibody productivity as the freely suspended cells. These capsules could withstand severe agitation and aeration in an air-lift reactor over a period of 3 weeks with minimal damage.