Soluble membrane antigens of human malignant melanoma cells

✍ Scribed by Ariel C. Hollinshead; Ronald B. Herberman; William J. Jaffurs; Louis K. Alpert; John P. Minton; Jules E. Harris

• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: English
• Weight: 666 KB
• Volume: 34
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(197410)34:4<1235::aid-cncr2820340433>3.0.co;2-k

✦ Synopsis

Delayed hypersensitivity reactions to soluble components of the cell membranes of autologous a n d allogeneic tumors were elicited in a series of patients with malignant melanoma. T w o skin reactive antigens were prepared using stepwise low frequency sonication of cell membranes, chromatography of membrane sonicates on Sephadex G-200, and separation by polyacrylamide gel electrophoresis (PAGE). One group of antigens, i n Sephadex fraction 11, PAGE region a, appears to b e melanoma-associated antigen. Sephadex fraction 11, a n d further separated Sephadex fraction I1 PAGE region a, produced no reactions in 21 of 22 tests i n patients with cancers other than melanoma; positive reactions were seen in 17 of 22 patients with early stage melanoma a n d in 7 of 19 patients with late stage melanoma. The other antigen, from Sephadex fraction 111, PAGE region b, was also reactive i n 5 of 6 early stage breast cancer patients. Comparable separated proteins from Sephadex fraction 111, and Sephadex fraction 111 PAGE region b, of normal black skin cell membrane sonicates were reactive i n 4 of 9 early stage melanoma patients but were negative in 7 late stage melanoma patients. Sephadex fraction I11 a n d Sephadex fraction I11 PAGE region b produced no reactions i n patients with renal, cervical, colonic, a n d head a n d neck cancer; 9 of 21 patients with early stage melanomas were skin test positive; positive reactions were seen in 13 of 18 patients with late stage melanoma.