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Solubilization, partial purification and properties of lipoxygenase from apples

✍ Scribed by Marcel Feys; René De Mot; Willy Naesens; Paul Tobback

Book ID
112540530
Publisher
Springer
Year
1982
Tongue
English
Weight
587 KB
Volume
174
Category
Article
ISSN
0044-3026

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Partial purification and some properties
Partial purification and some properties of lipoxygenase fromSaccharomyces cerevisiae
✍ B. Bisakowski; S. Kermasha; C. Schuepp 📂 Article 📅 1995 🏛 Springer 🌐 English ⚖ 236 KB

A partially purified lipoxygenase extract was obtained from the yeast Saccharomyces cerevisiae by precipitation with solid (NH4)SO4 at 20% to 80% saturation. The enzyme had two pH optima, at pH 8.0 and 10.0, with respective apparent K m values of 13 and 9.5 μM. At both pH optima, the lipoxygenase de