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Solubilization of Escherichia coli Recombinant Proteins from Inclusion Bodies

โœ Scribed by Simpson, R. J.


Book ID
115485596
Publisher
Cold Spring Harbor Laboratory Press
Year
2010
Tongue
English
Weight
76 KB
Volume
2010
Category
Article
ISSN
1940-3402

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Overexpression of cloned or synthetic genes in Escherichia coli often results in the formation of insoluble protein inclusion bodies. Within the last decade, specific methods and strategies have been developed for preparing active recombinant proteins from these inclusion bodies. Usually, the inclus