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Efficient refolding of recombinant lipase from Escherichia coli inclusion bodies by response surface methodology

✍ Scribed by Neda Akbari; Khosro Khajeh; Nasser Ghaemi; Zahra Salemi

Book ID: 116829074
Publisher: Elsevier Science
Year: 2010
Tongue: English
Weight: 693 KB
Volume: 70
Category: Article
ISSN: 1046-5928
DOI: 10.1016/j.pep.2009.10.009

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## Abstract Response surface methodology was used to optimize 1,3‐propanediol production by a novel recombinant __Escherichia coli__ JM109 (pHsh‐__dha__B‐__yqh__D). The optimal fermentation parameters for enhanced 1,3‐propanediol yield were found to be: glycerol 61.8 g L^−1^, yeast extract 6.2 g L^