Optimization of 1,3-propanediol production by novel recombinant Escherichia coli using response surface methodology

✍ Scribed by Xiaomei Zhang; Yin Li; Bin Zhuge; Xueming Tang; Wei Shen; Zhiming Rao; Huiying Fang; Jian Zhuge

• Publisher: Wiley (John Wiley & Sons)
• Year: 2006
• Tongue: English
• Weight: 115 KB
• Volume: 81
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/jctb.1538

✦ Synopsis

Abstract

Response surface methodology was used to optimize 1,3‐propanediol production by a novel recombinant Escherichia coli JM109 (pHsh‐__dha__B‐__yqh__D). The optimal fermentation parameters for enhanced 1,3‐propanediol yield were found to be: glycerol 61.8 g L^−1^, yeast extract 6.2 g L^−1^, Vitamin B~12~ 0.049 g L^−1^ and fermentation time 30 h. Subsequent experimental trials confirmed the validity of the model. These optimal fermentation conditions in the cultivation flask culture led to a 1,3‐propanediol concentration of 43.1 g L^−1^ and a conversion rate of 69.7% (g g^−1^). A maximum 1,3‐propanediol concentration of 41.1 g L^−1^ was achieved in a 5 L fermenter using the optimized parameters. Copyright © 2006 Society of Chemical Industry