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Crossflow filtration for the separation of inclusion bodies from soluble proteins in recombinant escherichia coli cell lysate

✍ Scribed by S.M. Forman; E.R. DeBernardez; R.S. Feldberg; R.W. Swartz


Book ID
116130757
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
887 KB
Volume
48
Category
Article
ISSN
0376-7388

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In previous parts of this study we developed procedures for the high-efficiency chemical extraction of soluble and insoluble protein from intact Escherichia coli cells. Although high yields were obtained, extraction of recombinant protein directly from cytoplasmic inclusion bodies led to low product