Crossflow filtration for the separation of inclusion bodies from soluble proteins in recombinant escherichia coli cell lysate
β Scribed by S.M. Forman; E.R. DeBernardez; R.S. Feldberg; R.W. Swartz
- Book ID
- 116130757
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 887 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0376-7388
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A method is presented for the direct extraction of the recombinant protein Long-R 3 -IGF-I from inclusion bodies located in the cytoplasm of intact Escherichia coli cells. Chemical treatment with 6M urea, 3 mM EDTA, and 20 mM dithiothreitol (DTT) at pH 9.0 proved an effective combination for extract
In previous parts of this study we developed procedures for the high-efficiency chemical extraction of soluble and insoluble protein from intact Escherichia coli cells. Although high yields were obtained, extraction of recombinant protein directly from cytoplasmic inclusion bodies led to low product