A method is presented for the direct extraction of the recombinant protein Long-R 3 -IGF-I from inclusion bodies located in the cytoplasm of intact Escherichia coli cells. Chemical treatment with 6M urea, 3 mM EDTA, and 20 mM dithiothreitol (DTT) at pH 9.0 proved an effective combination for extract
โฆ LIBER โฆ
Preparation and characterization of recombinant protein ScFv(CD11c)-TRP2 for tumor therapy from inclusion bodies in Escherichia coli
โ Scribed by Geng Kou; Shu Shi; Hao Wang; Min Tan; Jingya Xue; Dapeng Zhang; Sheng Hou; Weizhu Qian; Shuhui Wang; Jianxin Dai; Bohua Li; Yajun Guo
- Book ID
- 116828472
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 505 KB
- Volume
- 52
- Category
- Article
- ISSN
- 1046-5928
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