𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Solubilization and partial characterization of a tumor-rejection antigen from an ultraviolet light-induced murine tumor

✍ Scribed by Janet H. Ransom; Cara-Lynne Schengrund; Gerald L. Bartlett

Publisher
John Wiley and Sons
Year
1981
Tongue
French
Weight
950 KB
Volume
27
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Tumor rejection antigen (TRA) of an ultraviolet‐light‐induced murine skin tumor was solubilized, fractionated and partially characterized. Subcellular fractions were prepared by differential centrifugation of tumor cells that had been ruptured via nitrogen cavitation. Only the 110,000‐g membrane fraction induced significant tumor protection, as determined by in vivo immunization and challenge assays. Extraction of the membrane fraction with 3 M KCI resulted in solubilization of material that could induce in vivo tumor‐rejection immunity. Both the membrane fraction and soluble extract had a limited effective dose range. The KCI extract was separated on a Sepharose, CL‐6B column in the presence of 6 M guanidine‐HCI. Only one of five fraction pools (molecular weight range of 76,000–127,000 daltons) was immunogenic. It contained at least eight protein bands by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE), but no lipid components. This immunogenic Sepharose fraction was chromatographed on a Sephadex G‐150 column. Each of the four Sephadex fraction pools was immunogenic. One protein component was common to each of those fractions. It migrated as a single 76,000‐dalton band on SDS‐PAGE and contained [^14^C]‐leucine and [^3^H]‐glucosamine that had been incorporated during cell growth. These results suggest that the TRA of this tumor is a 76,000‐dalton glycoprotein.

📜 SIMILAR VOLUMES

Immunogenic properties of a soluble tumo
Immunogenic properties of a soluble tumor rejection antigen (TSTA) from a simian virus 40-induced sarcoma
✍ Michael J. Rogers; Lloyd W. Law 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 French ⚖ 736 KB

## Abstract Tumor rejection antigen (TSTA) has been prepared in soluble form and in good yield from the tissue‐culture‐adapted SV40‐induced sarcoma, mKSA, without resorting to the usual extraction procedures of proteolysis, 3 M KCI extraction or treatment with detergents. Chromatography on a Biogel

Separation of the tumor rejection antige
Separation of the tumor rejection antigen (TSTA) from the major viral structural proteins associated with the membrane of an R-MuLV-induced leukemia
✍ Michael J. Rogers; Lloyd W. Law; Maria Prat; Stephen Oroszlan; Ettore Appella 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 French ⚖ 685 KB

## Abstract A tumor rejection antigen (TSTA) has been solubilized by DOC ^1^ from purified plasma membranes of an R‐MuLV‐induced leukemia, RBL‐5. Gel filtration chromatography resulted in the separation of high molecular weight fractions containing TSTA from lower molecular weight fractions contain

Biochemical characterization and biologi
Biochemical characterization and biologic activities of 82-and 86-kDa tumor antigens isolated from a methylcholanthrene-induced sarcoma, CII-7
✍ Garrett C. Dubois; Lloyd W. Law 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 French ⚖ 853 KB

Two tumor-specific antigens, with molecular weights of approximately 82 and 86 kDa, have been isolated and purified to apparent homogeneity from the methylcholanthrene-induced sarcoma, Cll-7. The method of purification used was essentially that previously employed for the isolation of the 82-and 86-

Isolation of a tumor-associated transpla
Isolation of a tumor-associated transplantation antigen (TATA) from an SV40-induced sarcoma. Resemblance to the TATA of chemically induced neoplasms
✍ Garrett C. Dubois; Ettore Appella; Lloyd W. Law 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 French ⚖ 568 KB

A tumor rejection antigen (TATA), obtained from the cytosol of a BALB/c mKSA sarcoma induced by SV40 virus, has been partially purified. This partially purified antigen is strikingly immunogenic against mKSA, providing more than 90% inhibition of growth at levels of 10-30 pg. This antigen preparatio

Some immunogenic and biochemical propert
Some immunogenic and biochemical properties of tumor-associated transplantation antigens (TATA) obtained in soluble form or solubilized from two methylcholanthrene-induced sarcomas, meth A and CI-4
✍ Michael J. Rogers; Lloyd W. Law 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 French ⚖ 718 KB

## Abstract The subcellular distribution of the tumor‐associated transplantation antigens (TATAs) of two highly immunogenic methylcholanthrene‐induced sarcomas, Meth A and CI‐4, were compared. Most of the TATA of CI‐4 was found in the soluble fraction (cytosol) of the cell while the TATA from Meth