✦ LIBER ✦

Separation of the tumor rejection antigen (TSTA) from the major viral structural proteins associated with the membrane of an R-MuLV-induced leukemia

✍ Scribed by Michael J. Rogers; Lloyd W. Law; Maria Prat; Stephen Oroszlan; Ettore Appella

Publisher: John Wiley and Sons
Year: 1978
Tongue: French
Weight: 685 KB
Volume: 21
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910210218

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A tumor rejection antigen (TSTA) has been solubilized by DOC ^1^ from purified plasma membranes of an R‐MuLV‐induced leukemia, RBL‐5. Gel filtration chromatography resulted in the separation of high molecular weight fractions containing TSTA from lower molecular weight fractions containing the viral structural proteins, gp70 and p30. Furthermore, the antigen reacting with a syngeneic serum from mice immunized with RBL‐5 membranes was also separated from TSTA and evidence is presented that the antigen recognized by this serum is the viral envelope protein p15E. The implications of these results for the host anti‐tumor immune response are discussed.

📜 SIMILAR VOLUMES

Separation of the moloney leukemia virus

Separation of the moloney leukemia virus-determined cell surface antigen (MCSA) from known virion proteins associated with the cell membrane

✍ Wolfgang Siegert; Eva Maria Fenyö; George Klein 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 705 KB

## Abstract Cell membranes of Moloney lymphoma cells (YAC, of strain A origin) were solubilized by NP40. The antigenicity of the solubilized protein fraction was assayed by inhibition of the corresponding cytotoxic reaction against YAC target cells. The Moloney leukemia virus (MLV)‐determined cell