Solid phase synthesis of 5'-phosphate labelled polynucleotides
β Scribed by Lorenzo De Napoli; Luciano Mayol; Gennaro Piccialli; Ciro Santacroce
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- French
- Weight
- 529 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0040-4020
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β¦ Synopsis
The solid phase synthesis of non-radioactive 5'-phosphate labelled polydeoxyribonucleotides is described, based on a single polymeric support prefunctionalized with a marking molecule.
π SIMILAR VOLUMES
I4C-Labeled inosine 5'-phosphate was synthesized by direct phosphorylation of i n 0 s i n e -8 -~~C with phosphoryl chloride followed by hydrolysis of the resulting phosphorodichloridate. Isolation and recrystallization o f its disodium salt afforded pure material in 66.8% overall chemical yield.
A new route leading to a simple and rapid procedure employing Methyl Phosphoroditetrazolide (MPDT) as condensing agent for polynucleotide synthesis is described. The phosphite triester procedure, introduced by Letsinger (l), has proven to be a general procedure for the synthesis of oligonucleotides
## Abstract An efficient and reliable multiβstep synthesis of 251 natural productβlike [5.5]βspiroketals on solid supports has been developed. As central key step, a double intramolecular heteroβMichael (DIHMA) reaction to alkynones was applied. The sequence allows for introduction of numerous subs